Archives of Industrial Biotechnology

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Editorial - Archives of Industrial Biotechnology (2024) Volume 8, Issue 6

Advances in antibody engineering for therapeutic applications.

Isla Foster *

School of Biological Sciences, University of Edinburgh, UK

*Corresponding Author:
Tim Keller
School of Biological Sciences, University of Edinburgh, UK
E-mail: tk12@tum.de

Received: 02-Dec-2024, Manuscript No. AAAIB-24-154692; Editor assigned: 03-Dec-2024, PreQC No AAAIB-24-154692 (PQ) Reviewed:17-Dec-2024, QC No. AAAIB-24-154692 Revised:24-Dec-2024, Manuscript No. AAAIB-24-154692 (R); Published:30-Dec-2024, DOI:10.35841/aaaib-8.6.244

Citation: Foster I. Advances in antibody engineering for therapeutic applications. Arch Ind Biot. 2024;8(6):244

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Introduction

Antibody-based therapeutics have revolutionized the treatment of various diseases, particularly cancer, autoimmune disorders, and infections. Over the last few decades, significant advances in antibody engineering have expanded their potential applications and improved their efficacy. These engineered antibodies, which are designed to target specific molecules or cells, hold promise for developing next-generation therapies with greater precision, efficacy, and safety. This article explores the recent advances in antibody engineering and their impact on therapeutic applications [1].

Monoclonal antibodies (mAbs) emerged as one of the most successful therapeutic interventions since their development in the 1970s. These antibodies are derived from a single B-cell clone, allowing for the production of antibodies with a high degree of specificity. In recent years, mAbs have become a cornerstone of cancer therapy, with drugs like rituximab and trastuzumab leading the way. Advances in hybridoma technology, phage display, and humanization techniques have further refined the design of mAbs, improving their efficacy while reducing immunogenicity [2].

A significant leap in antibody engineering is the development of bispecific antibodies (BsAbs), which are designed to recognize and bind to two different antigens simultaneously. BsAbs offer great potential in cancer therapy, particularly in redirecting immune cells to tumor cells. These antibodies can enhance immune cell engagement with tumor cells, leading to more effective immune responses. The advent of bispecific T-cell engagers (BiTEs), such as blinatumomab, has provided new options for the treatment of hematological malignancies like leukemia and lymphoma [3].

Antibody-drug conjugates (ADCs) represent another breakthrough in antibody engineering. These agents combine the specificity of monoclonal antibodies with the cytotoxic potential of chemotherapy agents. ADCs target cancer cells with minimal off-target effects, delivering potent drugs directly to tumor tissues. Advances in linker technology and drug payloads have greatly improved the therapeutic index of ADCs. Drugs like ado-trastuzumab emtansine have demonstrated improved outcomes in treating HER2-positive breast cancer [4].

The effector functions of antibodies, such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), play a critical role in their therapeutic effectiveness. Fc engineering has become a major focus in antibody development, as modifying the Fc region can enhance these effector functions. By optimizing the interaction between the antibody’s Fc region and immune cells, researchers have developed antibodies that can more effectively trigger immune responses, leading to improved anti-tumor and anti-viral effects [5].

Bispecific T-cell engagers (BiTEs) have emerged as a powerful class of therapeutic antibodies that bring T-cells into close contact with tumor cells. These antibodies are designed to bind simultaneously to a tumor-specific antigen and CD3 on T-cells, effectively redirecting T-cells to destroy the tumor. BiTEs have shown promising results in clinical trials for treating hematological cancers, such as acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma, with the success of blinatumomab paving the way for future therapies [6].

Another exciting development is the creation of immunomodulatory antibodies, which enhance the immune system’s ability to recognize and attack cancer cells. Immune checkpoint inhibitors, such as pembrolizumab and nivolumab, which block immune checkpoints like PD-1/PD-L1, have revolutionized the treatment of cancers such as melanoma and non-small cell lung cancer (NSCLC). Research in this area continues to expand, with new targets and combination therapies under investigation to overcome immune evasion by tumors [7].

Historically, many therapeutic antibodies were derived from animal sources, which led to immune responses in human patients. The humanization of antibodies, a process that replaces most of the non-human components with human sequences, has addressed this issue. Fully human antibodies, generated through phage display or transgenic mice, offer a solution to the immunogenicity problem and have become the gold standard in the development of therapeutic antibodies. This innovation has led to the approval of multiple antibodies with better safety profiles and efficacy [8].

While traditional monoclonal antibodies remain a mainstay in therapy, next-generation antibody formats are gaining traction. Single-domain antibodies (sdAbs), such as nanobodies derived from camelids, offer unique advantages due to their small size, stability, and ease of production. These antibodies can be engineered to bind to targets that are challenging for traditional antibodies. Other emerging formats include antibody fragments (Fab, scFv) and engineered scaffold proteins, which provide additional versatility in targeting diseases [9].

Personalized medicine is increasingly becoming a focus in the field of antibody therapeutics. By tailoring treatments to the specific characteristics of an individual's disease, personalized antibody therapies aim to improve treatment outcomes and minimize side effects. Genetic profiling and tumor sequencing have enabled the identification of specific biomarkers that guide the selection of the most appropriate antibody therapies. This approach has shown success in cancers like breast and lung cancer, where targeted therapies based on genetic mutations can dramatically improve patient outcomes [10].

Conclusion

Advances in antibody engineering have greatly expanded the therapeutic potential of antibodies, leading to new and more effective treatments for a range of diseases. From monoclonal antibodies to bispecifics, ADCs, and immune checkpoint inhibitors, the landscape of antibody-based therapies is evolving rapidly. With continued research and innovation, the future of antibody engineering promises even greater strides in the treatment of cancer, autoimmune diseases, and infectious conditions, ultimately improving patient outcomes and quality of life.

References

  1. Kandari D, Bhatnagar R. Antibody engineering and its therapeutic applications. Int Rev Immunol. 2023;42(2):156-83.

    2. Indexed at, Google Scholar, Cross Ref

  2. Kim SJ, Park Y, Hong HJ. Antibody engineering for the development of therapeutic antibodies. Mol Cells. 2005;20(1):17-29.

    3. Indexed at, Google Scholar, Cross Ref

  3. Filpula D. Antibody engineering and modification technologies. Biomol Eng. 2007;24(2):201-15.

    4. Indexed at, Google Scholar, Cross Ref

  4. Jain M, Kamal N, Batra SK. Engineering antibodies for clinical applications. Trends Biotechnol. 2007;25(7):307-16.

    5. Indexed at, Google Scholar, Cross Ref

  5. Schmid AS, Neri D. Advances in antibody engineering for rheumatic diseases. Nat Rev Rheumatol. 2019;15(4):197-207.

    6. Indexed at, Google Scholar, Cross Ref

  6. Chiu ML, Gilliland GL. Engineering antibody therapeutics. Curr Opin Struct Biol. 2016;38:163-73.

    7. Indexed at, Google Scholar, Cross Ref

  7. Presta LG. Selection, design, and engineering of therapeutic antibodies. J Allergy Clin Immunol. 2005;116(4):731-6.

    8. Indexed at, Google Scholar, Cross Ref

  8. Tiller KE, Tessier PM. Advances in antibody design. Annu Rev Biomed Eng. 2015;17(1):191-216.

    9. Indexed at, Google Scholar, Cross Ref

  9. Raja A, Kasana A, Verma V. Next-Generation Therapeutic Antibodies for Cancer Treatment: Advancements, Applications, and Challenges. Mol Biotechnol. 2024:1-21.

    10. Indexed at, Google Scholar, Cross Ref

  10. Saeed AF, Wang R, Ling S, et al. Antibody engineering for pursuing a healthier future. FrontMicrobiol. 2017;8:495.

    11. Indexed at, Google Scholar, Cross Ref

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