Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Vitamin C and E attenuates diabetic nephropathy through the regulation of IGF-1 and regucalcin mRNA expression in PCB exposed experimental rats.

Background: Deterioration of kidney mainly due to diabetes results in causing diabetic nephropathy. PCB is a group of chemical compounds and is one of the halogenated aromatic pollutants which have caused diverse effects on the environment. Aim: To study the effect of Vitamin C and E in the expression of regucalcin and IGF-1 in Polychlorinated Biphenyls (PCB) induced diabetic nephropathy. Methods: Male albino Wistar rats were used for the study and they were divided in 3 groups. Group 1: Control; Group 2: Rats received PCB intraperitoneally at a dose of 2mg/kg b.wt; Group 3: PCB induced rats were treated with Vitamin C (100mg/kg b.wt) and vitamin E (50 mg/kg b.wt) through gastric intubation. After the experimental period animals were sacrificed and the kidney was taken for further studies. The data were analyzed statistically by one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test and it was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Results: The real time PCR analysis showed that Insulin like growth factor mRNA level is significantly increased on PCB induction, while it is significantly decreased on Vitamin C and E supplementation. The expression of regucalcin shows no significant changes in all the 3 groups. The data were analysed statistically by a one way Analysis of Variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Conclusion: From previous studies we have observed that PCB increases the oxidative stress which leads to inflammation. Vitamin C and E inhibits the mechanism of signalling. In this study, we can conclude that PCB exposure induces diabetic nephropathy suggesting that PCB has adverse effects on the kidney. This study suggests that antioxidant vitamins have the potential role by normalising the levels of IGF and regucalcin.

Author(s): Snigdha Bharadwaz, Gayathri R, V Vishnu Priya, J Selvaraj, Kavitha S*

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