Review Article - Journal of Biochemistry and Biotechnology (2025) Volume 8, Issue 1
Characterization, Partial purification and Optimization of Cellulase enzyme production from bacteria isolated from mangrove soil of Bhitarkanika, Odisha
The mangroves of Bhitarkanika, Odisha, harbor a diverse microbial population, including cellulose-degrading, phosphate-solubilizing, sulfur-oxidizing, and reducing bacteria. Among these, cellulose-degrading bacteria are dominant due to the nutrient-rich soil. Cellulases are in high demand for converting lignocellulosic materials into renewable energy, chemicals, and biofuels. This study aimed to isolate cellulolytic bacteria from Bhitarkanika mangrove soil and assess their cellulase production using CMC agar. Out of 20 bacterial isolates, 10 demonstrated cellulase activity, ranging from 0.014±0.004 to 0.267±0.007. The isolate BCDB10, showing high cellulolytic activity, was optimized for enzyme production using response surface methodology (RSM). The enzyme was purified through ammonium sulfate precipitation and DEAE-cellulose chromatography, revealing a molecular mass of 54 kDa. Phenotypic characterization identified BCDB10 as a Gram-positive, non-motile, spore-forming rod, presumptively Bacillus. While BCDB10 exhibited significant cellulase activity, further studies are needed to evaluate its industrial potential for cellulase production.
Author(s):Swetlina Sahoo, Amisha Biswal, Priyanka Prusty