Research Article - Biomedical Research (2017) Volume 28, Issue 8

Metabolomics analysis of human pancreatic cancer tissue and paired adjacent tissue samples

Background/aims: This study aimed to apply ¹H spectrum Nuclear Magnetic Resonance spectroscopy (1H-NMR) to detect the metabolites in pancreatic cancer tissue and paired adjacent tissue samples.

Methods: 48 samples were taken from cancer tissues of 24 pancreatic cancer patients and paired adjacent tissues. The samples were subjected to 1H-NMR and principle component analysis to compare endogenous small-molecule metabolites.

Results: Leucine and isoleucine levels were significantly higher in pancreatic cancer tissues than in control tissues. Lactate, betaine, choline, α-glucose and α-hydroxybutyrate levels were significantly lower in pancreatitic cancer tissues than in control tissues.

Conclusions: ¹H-NMR-based metabolomics is an effective method to identify small-molecule metabolites in pancreatic cancer tissues. These metabolites can be used as the metabolic markers of pancreatic cancer for early diagnosis.

Author(s): Dong Ouyang, Qingyue Dai

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