Research Article - Biomedical Research (2017) Volume 28, Issue 1

Isolation and characterization of novel Tibetan Macaque (Macaca thibetana) microsatellite loci: cross-species amplification and population genetic applications

Aims: To investigate novel microsatellite loci of Tibetan Macaque (Macaca thibetana) and Simple Sequence Repeat (SSR) loci polymorphism in parent population of 30 pairs human and Tibetan Macaques.

Methods: Fluorescence labeling primer PCR and Capillary Electrophoresis were used to detect the Simple Sequence Repeat (SSR) loci polymorphism in parent population of 30 pairs human and Tibetan Macaques.

Result: Results showed that total 12 polymorphism loci were screened out, of which, the Polymorphic information content (PIC) and Expected heterozygosity (HExp) of M. thibetana parent population were 0.718 and 0.761, respectively. The exclude cumulative individual rate was 0.99999 (CNE-I). The average probability that the set of loci was not an unrelated candidate parent from parentage of an arbitrary offspring when the genotype of the other parent was unknown (CNE-1P) and the average probability that the set of loci will not exclude an unrelated candidate parent from parentage of an arbitrary offspring when the genotype of the other parent is known (CNE-2P) were 0.9968 and 0.9999; the genetic diversity of filial generation was slightly lower than that of parents.

Conclusion: The 12 polymorphism loci in M. thibetana were useful for the specific individual identification, effective paternity test and accurate genetic testing.

Author(s): Na Yang, Liang Zhou, Xiaoshuai Liu, Daiwen Zeng

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