Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Impact of glyphosate on nuclear factor erythroid 2 - related factor 2 and heme - oxygenase expression in liver of adult male rats

Glyphosate is a compound which is a herbicide and it is harmful to animals and also to plants when used in large amounts. Nrf2 is an emergency regulator of cellular resistance towards oxidants. Hemeoxygenase catalyses heme through heme oxygenase system. To analyse the impact of glyphosate on Nrf2 and heme-oxygenase expression in the liver of adult male rats. Male albino rats of Wistar strain weighing 180g-210g were used in this study. The rats were divided into 4 groups, each consisting of 6 animals. The animals were sacrificed and the liver tissue was taken to do real time PCR analysis for Nrf2 and HO. Data were analysed by ANOVA and DUNCAN’S multiple range test to check the statically significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Glyphosate-induced rats showed a significant (p<0.05) reduction in the expression of Heme oxygenase-1 and Nrf2 mRNA compared to normal control rats in a dosedependent manner suggesting that glyphosate induces diabetes by downregulating Nrf2 mediated signaling in liver. Glyphosate exposure has detrimental changes in downregulating the expression of Nrf2 signaling molecules and thereby it induced diabetes by disrupting antioxidant signaling.

Author(s): Danda Nishitha, Gayathri R, V Vishnu priya, J Selvaraj, Kavitha S

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