Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Effect of diosmin on the expression of E-cadherin,vimentin and beta catenin in the liver of NDEA-induced experimental rats

Background: Liver cancer or Hepatocellular Carcinoma (HCC) is a very common primary liver malignancy and is one of the leading causes of cancer-related deaths globally. Diosmin is a citrus flavonoid that's naturally obtained from flavone glycoside. It is known to have anticancer properties. Aim: To study the effect of diosmin on the expression of E-cadherin, vimentin and beta catenin in the liver of NDEA induced experimental rats. Methods: Healthy male albino rats were divided into three groups. First one being the control group, the second one being the NDEA induced group and the third one being the diosmin treated group. The animals were then sacrificed and the tissues were excised. Then the RNA was isolated and it was converted to cDNA using a reverse transcriptase enzyme. The obtained results were plotted using quantitative Real Time Polymerase Chain Reaction (RT-PCR) on a graph. The data were analysed statistically by one way Analysis of Variance (ANOVA) followed by Duncan’s multiple range test that was used to see the statistical significance among the groups. Results with p<0.05 level were considered to be statistically significant. Results: In this study it was observed that NDEA induced detrimental changes on the expression of beta catenin and e cadherin signalling molecules was reversed by diosmin treatment stating that diosmin has a significant role in controlling these signalling molecules. Conclusion: Present findings conclude that diosmin has anticancer properties by modulating the expression of beta catenin/ E-cadherin signalling molecules and thus it can be used as a natural flavonoid in combating liver cancer.

Author(s): Ridha Azimudin, Gayathri R, V Vishnu Priya, J Selvaraj, Kavitha S

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