Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Effect of diosmin on P13K and AKT expression on NDEA induced hepatocellular cellular carcinoma in rats - an in vitro study.

Introduction: Diosmin is a flavone found in Teucrium gnaphalodes and in some citrus fruits indicated for the treatment of venous disease. Dismosin is also used to hemorrhoids and leg sores caused due to poor blood flow. Diosmin is not recommended for use in children or women during pregnancy. Diosmin is not recommended for treating the rectal mucosa, skin irritations, or wounds, and should not be used to treat dermatitis, eczema or urticaria. The oral administration of diosmin as a protective agent normalized the altered levels of AFP, LPO, antioxidant enzymes, pro and anti-apoptotic proteins as well as caspase-3 and -9 proteins. In a previous study, desmosin is a flavanone glycoside that is found in the citrus species and showed antioxidant, hepatoprotective as well as anticancer activity. Previous study on diosmin reveals that it acts as an anti-diabetic drug in type -2 diabetes mellitus Also acts as a potent anti-inflammatory drug. Pi3k gene is a master regulatory gene for cancer. Activation of the pi3k pathway leads to the development of tumors and gives resistance to anticancer therapeutic drugs. Phosphatidylinositol 3-Kinase (PI3K)–AKT signaling pathway regulates the expression of several downstream target genes that inhibit apoptosis and promote cell proliferation. The effect of its deregulation on the clinical outcome of human tumors has recently become a subject of intense investigation. Activation of this pathway has been associated with aggressive phenotype and poor prognosis in brain tumors (glioblastoma and neuroblastoma) as well as in various carcinomas, including breast, prostate, bladder, colon, and lung carcinomas Aim: To determine the effect of diosmin on Pi3k and Akt expression in NDEA induced hepatocellular carcinoma in experimental rats. Materials and methods: The present experimental study was approved by the institutional animal ethics committee. Adult male Wistar albino rats were obtained and maintained in clean propylene cages in an air-conditioned animal house, fed with standard rat pelleted diet orally for 16 weeks. At the end of the treatment, animals were anesthetized and blood was collected through cardiac puncture, sera were separated and stored. Gastrocnemius muscle from control and experimental animals was immediately dissected out and used for assessing the various parameters. Hepatic carcinoma by administering (NDEA) and the changes are then assessed using real time PCR. Results and discussion: Pi3k /AKT pathway is an intra-cellular signalling pathway important in regulating the cell cycle. Therefore, it is directly related to cellular quiescence proliferation cancer and longevity DIRK or AKT mRNA. In the current study there was a Dec 2 animals indicating the induction of hepatocellular cancer, whereas in the treatment group (group 3 treated with the diosmin) there is significant increase in the expression of PI3K or Akt which was equivalent to control oxidative stress. A similar study on cioskinagnin proves to be effective in pulmonary syndrome. Conclusion: Animals treated with diasmin clearly exhibited an improvement in Hepatocellular cancer, thus diasmin can augment anti-cancer therapy.

Author(s): VA Muralidharan, R Gayathri, J Selvaraj, V Vishnu Priya

Abstract PDF