Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Diosmin attenuates NDEA-induced changes in the mRNA expression of stress signalling in the liver of adult male rats.

This study is designed based on the beneficial role of diosmin, a biologically active flavonoid. It is a citrus flavone with antioxidant and anti-inflammatory properties which underlays its protection against cardiac, hepatic and renal injuries. Hepatocellular carcinoma is the most common form of liver cancer that occurs in people with liver disease, particularly in people with chronic hepatitis B and C. To study the diosmin effects in N-Nitrosodiethylamine (NDEA)-induced rats and to evaluate the mRNA expression of stress signalling in the liver of adult male rats. Healthy male albino rats were divided into three groups. First one being the control group, the second one being the NDEA induced group and the third one being the diosmin treated group. The animals were then sacrificed and the tissues were excised. Then the RNA was isolated and it was converted to cDNA using a reverse transcriptase enzyme. The obtained results were plotted using quantitative Real Time Polymerase Chain Reaction (RT-PCR) on a graph. The data were analysed statistically by one way Analysis of Variance (ANOVA) followed by Duncan’s multiple range test that was used to see the statistical significance among the groups. Results with p<0.05 level were considered to be statistically significant. diosmin plays significant role on stress signaling molecules in liver cells and hence, it could be considered as an anti-cancer therapeutic natural drug for liver cancer.

Author(s): Prateeksha Moharana, Kavitha S*, Selvaraj J, V Vishnupriya, Gayathri R

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