+44-7360-538437- Biomedical Research (2012) Volume 23, Issue 4
Bonding of cholesterol oxides to functionalised multiwalled carbon nanotubes for in-vitro biosensor application
MWCNTs (multiwalled carbon nanotubes) were synthesized by chemical vapour deposition (CVD) and functionalised using Hcl and HNO3 at 80 °C. Their FTIR spectra revealed the characteristic peaks for carboxylic acid and alcoholic groups. Both unfunctionalized (MWCNTs) and functionalised multiwalled carbon nanotubes ( f-MWCNTs) were reacted with cholesterol oxidase using 1-ethyl-3-(3-dimethylaminopropyl) carbodimide and Nhydroxyl sulfo-succinimide.But bonding with cholesterol oxidase was established only with f- MWCNTs. Cholesterol oxidase bonded to f-MWCNTs was tested for cholesterol sensing using cyclic voltametry. It established interaction of cholesterol with cholesterol oxidase bonded to f- MWCNTs. Hence cholesterol oxidase bonded to f-MWCNTs could be a novel in-vitro cholesterol sensor in hyper lipidemia
Author(s): A. Malarvizhi and A. Pandurangan