Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0

Association of BRAF gene polymorphism with susceptibility to chronic periodontitis- a case control study

Periodontitis is a multifactorial disease of tooth supporting tissue that is caused by bacterial infection and increased immune response. T-cell proliferation plays an important role in host response to bacterial infection. BRAF is a positive regulator of T cell proliferation. The present study was aimed to evaluate the genetic association of BRAF gene polymorphism (rs10487888). A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using ARMS PCR technique. The genotype obtained based on the ARMS PCR pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the chi-square test. The risk associated with individual alleles or genotypes was calculated as the Odds Ratio (OR) with 95% confidence intervals. Statistical significance in all tests was determined at p < 0.05. The C allele was found to be more predominant in the study population than T allele. The present study denotes that BRAF gene polymorphism is not associated with CP in the study group analyzed.

Author(s): Karthikeyan Murthykumar, Jaiganesh Ramamurthy*, Vijayashree Priyadharsini J

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