Research Article - Journal of RNA and Genomics (2022) Volume 0, Issue 0

Assessing some immune parameters during asymptomatic infection with Entamoeba histolytica.

Background and Aim: Entamoeba histolytica a protozoan parasite with high prevalence rates in tropical and subtropical regions of underdeveloped countries. Cellular and humoral immune system involves during invasive amebiasis but there is limited information about the exact immune response during asymptomatic infections which comprise the majority of the infected people. Material and Methods: in a case-control study, serum from asymptomatic carriers of E. histolytica and healthy control individuals were collected, and then levels of IL-4, TNF-α, Ig G2 and Ig G4 were measured by ELISA. A structured questionnaire was filled out by each participant and statistically analysed using SPSS program. Results: The results of present study showed that the levels of TNF-α and IL-4 were higher but not significant in the positive group compared to the negative control group. While, serum Ig G2 and Ig G4 levels were significantly higher in positive E. histolytica group compared to negative control individuals. Ig G2 levels were significantly higher in low-income people and individuals who always eating outside home compared to middle-income people and individuals who never or sometime eat outside, respectively, among the negative control group. There were also significant variations among the positive group, higher levels of IL-4 and Ig G2 were seen in people who washed their vegetables and fruits than who were not; while lower levels of Ig G4 were reported in the same category of participants. Conclusions: The current study concludes that the parasite did not elicit cellular immune response but causes activation of humoral immune response against the asymptomatic E. histolytica isolates.

Author(s): Hawri Mustafa Bakr

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