Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2022) Volume 12, Issue 91

Antidiarrheal and immunomodulatory activities of probiotic lactobacillus species isolated from palm wine, fowl gut and rotten banana fruit.

This study characterized and evaluated the antidiarrhoeal and immunomodulatory effects of probiotic isolates from palm wine, rotten banana fruit, and fowl gut. The probiotic isolates were identified using Gram staining, colonial description, and biochemical reactions. The isolated probiotics were characterized by cultural characteristics on MRS agar and resistance to low pH. The probiotics were screened for anti-diarrheal and immunomodulatory activities. The gastrointestinal motility was also evaluated. Lactobacillus species were isolated from palm wine, fowl gut and rotten banana fruit sources. The antidiarrheal activity was time dependent. Percentage reduction of the mean weight of wet stool by fowl gut, palm wine, banana isolates and the standard drug were 23%, 39%, 36% and 67% respectively. Mean delay of onset diarrhea by distilled water, fowl gut, palm wine, banana isolates and loperamide were 63, 80, 95, 122, and 157 respectively. The test isolates caused a significant reduction in the distance traveled by charcoal meal. Percentage reduction by atropine, palm wine, banana, fowl gut isolates and distilled water are 32%, 40%, 53%, 66%, 93% respectively. The probiotic isolates possess anti motility, anti-secretory and immuno stimulatory properties and could be alternative to standard drugs for the management of diarrhea and prevention of the onset of infections.

Author(s): Chinedu J Ezeibe, Malachy C Ugwu*, Nonye T Ujam, David C Nwobodo, Chiamaka B Ugwu, Anthony A Attama

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