Research Article - Journal of RNA and Genomics (2022) Volume 0, Issue 0

Antibacterial Activity of Extract and Fractions of Red Algae (Gracilaria sp.) against Pseudomonas aeruginosa and Pseudomonas Multi-Drug Resistant (PAMR)

Microbial keratitis is an eye infection in severe cases, the cause of loss of vision caused by Pseudomonas aeruginosa. P. aeruginosa is one of the bacteria with increasing cases of resistance and is called Pseudomonas aeruginosa Multi Resistance (PAMR). This condition encourages the discovery of new drugs as antibacterials for infections caused by P. aeruginosa. Red algae (Gracilaria sp.) is known to have bioactive content as antimicrobials from the alkaloids, flavonoids, steroids, triterpenoids, and tannins. This study aimed to determine the antibacterial activity of ethanol extract and red algae fraction against P. aeruginosa and PAMR and to determine the Minimum Growth Inhibitory Concentration (MIC) and Minimum Kill Concentration (MBC) of the most active fraction. The method used was the agar diffusion method with paper discs and the determination of MGIC and MKC by microdilution. The results showed that the antibacterial activity of red algae was only found in the ethyl acetate fraction, with the most active inhibition zones being 14.67 mm against P. aeruginosa and 15.36 mm against PAMR. The MIC values resulting from the most active fraction were in the range of 25%-12.5% (w/v) for PAMR and 50%-25% for P. aeruginosa. From the results of the study, it can be concluded that the ethanol extract and the fraction of red algae (Gracilaria sp.) have antibacterial activity against both test microbial keratitis.

Author(s): R Mustarichie, S Sulistyaningsih, Y Iskandar

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