Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2024) Volume 14, Issue 104

ANALYSIS OF ALLELIC VARIANTS OF CYP2C19 IN GASTRIC BIOPSY SAMPLES

Helicobacter-related chronic gastritis is pathology with a high prevalence, it is considered the most common form of gastritis worldwide. The cornerstone of the therapy for this entity is proton pump inhibitors (PPIs), whose mechanism of action is the decrease in acid secretion. In general, this pharmacological group is characterized by a good profile, with a low frequency of adverse drug reactions. PPIs are metabolized by hepatic enzymes of the CYP2C19 family, which are encoded by a gene with polymorphisms that affect the way in which the drug is metabolized, implications that clinically mean the need for a higher or lower dose of the drug for optimal antisecretory effect and less development of adverse drug reactions. To analyze the allelic variants of CYP2C19 in patients with gastric disorders treated with PPIs. A descriptive, retrospective and observational research will be carried out. The analysis population are DNA samples from gastric biopsies stored in a DNA bank. A descriptive statistical analysis will be carried out through SPSS and the use of proton pump inhibitors and the diagnosis of gastric injury are applied as inclusion criteria. The determination of the alleles of the selected samples will be carried out through PCR amplification, subsequently they are subjected to digestion with restriction enzymes SmaI and BamH. The visualization of the restriction products will be carried out in agarose gels at 2% (w/v) stained with the GelRedTM dye. Contribute to the development of personalized medicine in patients with acid secretion disorders, treated with PPIs, thus improving the efficiency of therapy through the calibration of the dose according to the metabolizer phenotype.

Author(s): Sergio David Angulo*, David Cano , Cesar Augusto Cortes , Nicolas Jimenez , Alvaro Javier Rodriguez, Luis Fernando Perez , Paula Tatiana Uribe , Diego Alejandro Ruiz, Echeverry Jhon Fredy Betancur Perez

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